TO COMPARE THE EFFICACY OF HAEMATOXYLIN AND EOSIN (H AND E) AND PERIODIC ACID SCHIFF (PAS) TO IDENTIFY THE BREAK IN CONTINUITY OF BASEMENT MEMBRANE (BM) IN SQUAMOUS CELL CARCINOMA (SCC)
Authors/Creators
- 1. Saveetha Dental College and Hospitals, Chennai, Tamilnadu
Description
Aim: To analyse the efficacy of H and E and PAS to identify the break in continuity of basement membrane in SCC.
Objective: To evaluate the efficacy of H and E and PAS to identify the break in continuity of basement membrane in SCC.
Materials and Method: This entire study was conducted in Saveetha Dental College and Hospitals, Department of Oral Pathology.Five OSCC tissue samples were collected from the archives of Saveetha Dental College and Hospitals, sectioned by a microtome and two set of slides were prepared for each tissue sample. Then the first set of slides were stained with H and E and the other set of smears were stained with PAS following the staining protocol.
Back Ground: Basement membrane (BM) is an electromicroscopically, amorphous, thick sheet of extracellular matrix molecules, upon which epithelial cells attach. Squamous cell carcinoma (SCC) is an invasive epithelial neoplasm with varying degrees of squamous differentiation and a propensity to early and extensive lymph node metastases, occurring predominantly in alcohol and tobacco-using adults in the 5th and 6th decades of life. In SCC, there is a break in the basement membrane continuity.
Reason: Although the pathological changes in the basement membrane can be detected very specifically by immunohis to chemical methods on paraffin-embedded tissue sections, the antikeratin-antisera are not economical and are time consuming. Hence if the efficacy of the standard stains like H and E and special stains like PAS can be assessed and compared, it could be very advantageous because of its low cost and simple handling technique.
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Additional details
References
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