Dataset for the article "Beyond PLFA: Concurrent extraction of neutral and glycolipid fatty acids provides new insights into soil microbial communities"

The following are data and code used for statistical analysis and figure plotting in the manuscript

Gorka et al. (2023) "Beyond PLFA: Concurrent extraction of neutral and glycolipid fatty acids provides new insights into soil microbial communities", Soil Biology and Biochemistry

It contains the following files:

1. Pure lipid standard data

• Total ion chromatogram (TIC) area data (area.csv)
• Assignment of lipids that the measured fatty acids originate from (LipidClass.csv)
• An R script reproducing the calculations and plotting for Fig. 2 and Fig. S1 (pure_lipids.R)

2. Microbial pure culture fatty acid data data

• TIC area data of the PLFA, NLFA, and GLFA data from pure culture extracts (area.csv)
• Files needed for calculating the data and assigning taxonomic groups in the R code (weights.csv, C_atoms.csv, species_list.csv)
• An R script reproducing the calculations and plotting for Fig. 3, Fig. 4, Fig. S2, and Fig. S3 (pure_cultures.R)

3. Soil fatty acid data

• Absolute abundance data in nmol C g^-1 dry weight of the PLFA, NLFA, and GLFA data from soil extracts (nmolC.csv)
• Taxonomic group assignments of fatty acids needed to run the R code (phylum.csv)
• An R script reproducing the calculations and plotting for Fig. 5, and Fig. S4 (soil.R)