Investigation of Mesenchymal cells in the Microfluidic Cell Culture Device

Mesenchymal stem cells (MSCs), as multipotent stem cells, can be obtained from many tissues/organs including bonemarrow, adipose tissue, dental pulp, and amniotic or synovial fluids1. MSCs are unique cells that can support functionsof many tissues and organs by releasing soluble factors such as growth factors, anti-apoptotic and anti-inflammationfactors, and they can synthesize extracellular matrix (ECM) mainly composed of collagen fibrils, a key component oftissues. In damaged tissues, MSCs can help the formation of new blood vessels by secreting angiogenic factors.Therefore, MSCs are very helpful for reducing the inflammation2, decreasing the progress of autoimmune diseases3and preventing rejection of transplant4. Our objective in this study is to use MSCs as supportive/feeder cells for themaintenance of liver cells including cell adhesion, cell growth and proliferation other biological activity in microfluidicchip by benefitting from such protective and regenerative properties of MSCs for tissues. To accomplish this aim, aspart of a liver-on-a-chip study, we investigate the behavior of MSCs in microfluidic (MF) culture by monitoring cellviability, proliferation, cell numbers, and the expression of MSC-specific genes. Moreover, we will investigate the effectof MSCs on the viability and maintenance of liver cells within the MF-chip conducted by co-culture of MSCs withliver cells
(PDF) Investigation of Mesenchymal cells in the Microfluidic Cell Culture Device.