Cynanchum Acutum L: Phytochemical Screening, Allelopathic and Cyto/Genotoxicity Effects in the Plant Model Arachis Hypogaea
Authors/Creators
- 1. Department of Cellular and Molecular Biology, Faculty of Natural Science and Life, El Oued University, El Oued 39000, Algeria.
- 2. Laboratory of Biology, Environment and Health, Department of Biology, Faculty of Life and Natural Sciences, University of El Oued, 39000, Algeria
- 3. Department of Biology, Faculty of Natural Science and Life, El Oued University, El Oued 39000, Algeria.
- 4. Laboratory of Biodiversity and Application of Biotechnology in Agriculture, El Oued University, El Oued 39000, Algeria.
- 5. Laboratory of Applied Chemistry and Environment, Faculty of Exact Sciences University of Eloued, Algeria.
- 6. Laboratory of Extemophile Plants, Biotechnology Centre of Borj-Cedria, Hammam - Lif, Tunisia Environment and Health.
Description
Abstract
The plant Cynanchum acutum L. growing in the Algerian desert is a weed a climber, but it has been used in popular medicine. This plant is rich in biologically active compounds, so it can be valued and exploited to produce specific biopesticides. In this regard, the work aims to assess the allelopathic effect of the aqueous extract of the air part of Cynanchum ActumL through phytochemical screening, where the results showed that the plant contained (coumarins, ,saponins polyphenols, flavonoids, alcaloids, terpenes,tannins, quinones, aldehydes, and cardioglycoside ). Four levels of concentration were tested (control,15mg/ml, 30mg/ml and 45mg/ml) where the extract affected negatively on all indicators of germination and initial development of Arachis hypogaea L. And their value decreased linearly until it disappeared at a concentration of 45 mg/ml, and the percentage of inhibition linearly increased until it reached 100% at concentration 45 mg/ml with statistically significant differences recorded. This confirms the existence of an inhibition process that was confirmed by the microscopic study to detect the cytotoxicity of the seeds of Arachis hypogaea L. Treatment with an aqueous extract with a concentration that inhibits root growth 50%, which was extracted from the root growth equation, which showed a decrease in the mitotic index with the observation of chromosomal abnormalities such as (the beginning of the formation of 2 micronucleus at interphase, micronucleus at interphase, binucleated cells at interphase, disturbed at metaphase, stickiness at metaphase, oblique at metaphase, fragments at metaphase, bridge at anaphase, and binucleated cells diagonal).