Published November 10, 2023
| Version v1
Dataset
Open
CRISPR-Cas12a-integrated transgenes in genomic safe harbors retain high expression in human hematopoietic iPSC-derived lineages and primary cells
Authors/Creators
- 1. Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark
Description
We identified and characterized potential integration safe harbor sites (SHS) in human cells. Using the CRISPR-MAD7 system, we integrated transgenes at these genomic sites in iPSC, primary T and NK cells, and Jurkat cell line, and demonstrated efficient and stable expression at these loci. Subsequently, we validated the differentiation capabilities of engineered iPSC towards CD34+ hematopoietic stem and progenitor cells (HSPC), lymphoid progenitors (LPC), and natural killer (NK) cells, and showed that transgene expression was retained in these lineages.
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