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Published March 20, 2023 | Version 1.0.0

COverlap: a Fiji co-localization toolset (Supplementary material)

  • 1. INCIA

Description

Supplementary material for the COverlap toolset, a 4 steps Fiji/ImageJ toolset intended for the co-localization of two nuclear markers on multichannel Z-stack confocal images.

Testing Files.zip is a sample dataset that allows testing of the toolset.

protocol-IHC_DAPI405_Lectin488_EdU555_ERG633.xlsx is the immunohistochemistry protocol used to stain the 50µm rat brain slices on which the sample dataset images were acquired.

ImageAcquisition_Specs.docx contains the specifications of our image acquisition protocol (Microscopy checklist generated with  Rebecca Senft's app and FPbase spectra viewer)

Files

Testing Files.zip

Files (4.2 GB)

Name Size
md5:90074137e23b5e31ce46570e941b6fd3
19.2 kB Download
md5:94e9f463070528765998e267cd1ae029
18.4 kB Download
md5:67a2141986eb1759620818a74e5aa9d7
4.2 GB Preview Download

Additional details

Related works

Funding

Agence Nationale de la Recherche
UBGSNeuro - University of Bordeaux Neurocampus Graduate School ANR-17-EURE-0028
Centre National d'Études Spatiales
GraviBrain 4500072566