UPDATE: Zenodo migration postponed to Oct 13 from 06:00-08:00 UTC. Read the announcement.

Journal article Open Access

Reduction of Cell Proliferation by Acute C2H6O Exposure

Baldari, Silvia; Manni, Isabella; Di Rocco, Giuliana; Paolini, Francesca; Palermo, Belinda; Piaggio, Giulia; Toietta, Gabriele

Endogenous acetaldehyde production from the metabolism of ingested alcohol exposes hematopoietic progenitor cells to increased genotoxic risk. To develop possible therapeutic strategies to prevent or reverse alcohol abuse effects, it would be critical to determine the temporal progression of acute ethanol toxicity on progenitor cell numbers and proliferative status. We followed the variation of the cell proliferation rate in bone marrow and spleen in response to acute ethanol intoxication in the MITO-Luc mouse, in which NF-Y-dependent cell proliferation can be assessed in vivo by non-invasive bioluminescent imaging. One week after ethanol administration, bioluminescent signals in bone marrow and spleen decreased below the level corresponding to physiological proliferation, and they progressively resumed to pre-treatment values in approximately 4 weeks. Boosting acetaldehyde catabolism by administration of an aldehyde dehydrogenase activity activator or administration of polyphenols with antioxidant activity partially restored bone marrow cells' physiological proliferation. These results indicate that in this mouse model, bioluminescent alteration reflects the reduction of the physiological proliferation rate of bone marrow progenitor cells due to the toxic effect of aldehydes generated by alcohol oxidation. In summary, this study presents a novel view of the impact of acute alcohol intake on bone marrow cell proliferation in vivo.
Files (16.8 MB)
Name Size
16.8 MB Download
Views 30
Downloads 10
Data volume 167.8 MB
Unique views 24
Unique downloads 10


Cite as