Confocal microscopy imaging of FocalCheck fluorescent beads at various oil indices under ambient temperature

the dataset consisting of 20 fields of images with physical dimensions of 1340x1340x64 pixels. These images were acquired using two different refractive indices: one with oil immersion corrected for a temperature of 23°C and another with oil immersion corrected for a temperature of 37°C. A constant temperature of 24.5°C was maintained throughout the data collection