Imaging dataset 04 for mtFociCounter

This is the fourth dataset associated with the updated manuscript on mtFociCounter.

Images are Spinning Disk Confocal Images of unsorted U2OS cells treated with siRNA against TFAM (siTFAM) or neutral siRNA (siNT). Cells were stained with antibodies against tom20 (mitochondria) and against dsDNA (nucleoids).
The second folder contains SIM2 images acquired on an Elyra7 microscope of 3t3 wild-type fibroblasts, which express mitochondrially targeted dsRed, and are stained by immunofluorescence against dsDNA (nucleoids) and AlexaFluor 647, as well as Hoecst for nuclei.

This repository contains all data necessary to reproduce the analysis of siTFAM and 3t3-superresolution, as described in the manuscript. The dataset contains the following data:

U2OS treated with siTFAM or neutral (WT):
20210212 siTFAM vs. siNT
20210217 siTFAM vs. siNT
20210222 siTFAM vs. siNT

3t3 WT fibroblasts images by Elyra7 SIM2:
20221221
20230118
20230120

The third folder contains images from Spinning Disk Confocal Images of unsorted U2OS cells. Cells were stained with antibodies against tom20 (mitochondria) and against FASTKD2 (MRGs).
The fourth folder contains raw Western Blot and Coomassie staining images from unsorted U2OS cells treated with a neutral siRNA or an siRNA against TFAM for 3 days. Antibodies against tubulin, TFAM or pre-TFAM were used, as indicated.

This repository contains all data necessary to reproduce the analysis of MRG numbers or TFAM knockdown assessment, as described in the manuscript.

U2OS WT cells:
20230429 FASTKD2
20230505 FASTKD2
20230505 FASTKD2

For details of the experimental procedure, please refer to the accompanying manuscript, which will soon be made available on BioRxiv.