Published September 30, 2020
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Drought stress induces biosynthesis of flavonoids in leaves and saikosaponins in roots of Bupleurum chinense DC
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- 1. ∗ & ∗∗ & Cultivation Base of State Key Laboratory for Ecological Restoration and Ecosystem Management of Jilin Province and Ministry of Science and Technology, College of
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Yang, Lin-lin, Yang, Li, Yang, Xiao, Zhang, Tao, Lan, Yi-ming, Zhao, Yu, Han, Mei, Yang, Li-min (2020): Drought stress induces biosynthesis of flavonoids in leaves and saikosaponins in roots of Bupleurum chinense DC. Phytochemistry (112434) 177: 1-12, DOI: 10.1016/j.phytochem.2020.112434, URL: http://dx.doi.org/10.1016/j.phytochem.2020.112434
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- urn:lsid:plazi.org:pub:FFC8FFBDFFFEFFBCFFCFFFD2FFC5FF98
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- Concentrations of osmoregulatory substances (SP, PRO, and soluble sugars) and MDA (a product of membrane peroxidation) and enzymatic activities of SOD, POD, and CAT in B. chinense leaves were measured using a microplate reader (SpectraMax 190, Molecular Devices, USA). The specific detection methods were carried out as described in a previous study (Yang et al., 2019b). Activities of SOD, POD, and CAT are shown in units per milligram of fresh leaf weight.
- Total RNA was extracted from B. chinense leaves and roots using a Plant RNApure Kit (Zoman Biotechnology Co., Ltd.), according to the manufacturer's instructions. After total RNA agarose electrophoresis, A260/A280 absorbance ratios of total RNA were determined using a P330 nanophotometer (Thermo Fisher Scientific, USA) to assess RNA quality, and cDNA was produced using the PrimeScript™ RT Reagent Kit (Takara Biomedical Technology, (Beijing) Co., Ltd., China). The reaction system and procedures of quantitative reverse transcription PCR were described elsewhere (Cheng et al., 2018). Using EF-1α as a reference gene, relative gene expression levels of C4H, 4CL, IFS, F2H, and DFR in leaves were determined using an Mx 3000 P quantitative PCR instrument (Agilent, USA). The same procedure was applied for measuring relative gene expression levels of HMGR, IPPI, FPS, SS, SE, β- AS, P450-7, P450-12, and UGT-8 in roots. The respective PCR primers are shown in Table 5. Gene expression was calculated using the 2-ΔΔCt method (Livak and Schmittgen, 2001).