People's Journal of Scientific Research
Published June 1, 2012 | Version v1
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L-Asparaginase: A Promising Enzyme for Treatment of Acute Lymphoblastic Leukiemia

Authors/Creators

  • 1. Molecular Biotechnology Laboratory, Centre for Scientific Research & Development, Bhopal-462037

Description

Asparaginases are known to be the cornerstone for treatment of acute lymphoblastic leukemia (ALL) and are used for treatment in all pediatric regimens as well as in the majority of adult treatment protocols. Clinical hypersensitivity reactions against commercially available asparaginase have resulted in failure of asparaginase in treatment of ALL in more than 60% of cases. Thus, it is required to search for serologically different asparaginase from new organisms for the patients exhibiting sensitivity to one formulation of asparaginase, so that they can be switched to another to ensure that they receive the most efficacious treatment regimen possible. The present study report E. coli VRY-15, E. coli VRY-8 and E. coli VRY-14 as potent producer of L-asparaginase. The L-asparaginase obtained from E. coli VRY-15 showed highest specific activity i.e., 19.56 µmol/mg. Attempt was made to purify the enzyme. Molecular weight of purified L-asparaginase obtained from E. coli VRY-15 was found to be 56 K Da as determined using Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS- PAGE).

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References

  • 1. Amena S, Vishalakshi N, Prabhakar M, Dayanand A, Lingappa K: Production, purification and characterization of l-asparaginase from Streptomyces gulbargensis. Brazilian Journal of Microbiology, 2010;41(1): 173-178
  • 2. Basha NS, Rekha R, Komala M, Ruby S: Production of extracellular Anti-leukaemic enzyme L-asparaginase from Marine Actinomycetes by solid state and submerged fermentation: Purification and characterization. Tropical Journal of Pharmaceutical Research, 2009; 8 (4): 353-360.
  • 3. Boyse EA, Old LJ,Campbell HA, Mashburn LT: Suppression of murine leukemia's by L-asparaginase. Incidence of sensitivity among leukemias of various types: Comparative inhibitory activities of guinea pig serum L-asparaginase and Escherichia coli Lasparagianse. Journal of Experimental Medicine, 1967; 125(1):17-31.
  • 4. Campbell HA, Mashburn LT, Boyse EA, Old LJ: Two Lasparaginase from Escherichia-coli B. Their separation, purification and antitumor activity. Biochemistry, 1967; 6(3):721-730
  • 5. Cedar H, Schwartz JH: Production of L-asparaginase II by Escherichia coli. Journal of Bacteriology,1968; 96(6):2043-2048
  • 6. Clementi A: La desamidation enzymatique de l'asparagine chez les differéntes espéces animals et la signification physiologique de sa presence dans l'organisme. Archives of Physiology & Biochemistry, 1922; 19(4): 369-398.
  • 7. Cornea CP, Lupescu I, Vatafu I, Caraiani T, Savoiu VG, Campeanu Gh, Grebenisan I, Negulescu Gh.P, Constantinescu D: Production of L-asparaginase II by Recombinant Escherichia coli cells. Roumanian Biotechnology Letters, 2002; 7(3):717-722
  • 8. Davis BD, Mingioli ES: Mutants of Escherichia coli requiring methionine or vitamin B12. Journal of Bacteriology, 1950; 60(1):17-28.
  • 9. DeJong PJ: L-asparaginase production by Streptomyces griseus. Applied & Environmental Microbiology, 1972; 23 (6):1163-1164.
  • 10. Dhevagi P, Poorani E: Isolation and characterization of L-Asparaginase from marine actinomycetes. Indian Journal of Biotechnology, 2006; 5:514-520.
  • 11. El-Bessoumy A A, Sarhan M, Mansour J: Production, isolation, and purification of L-Asparaginase from Pseudomonas aeruginosa 50071 using solid-state fermentation. Journal of Biochemistry and Molecular Biology, 2004; 37(4):387-393.
  • 12. Gallagher MP, Marshall RD, Wilson, R: Asparaginase as a drug for treatment of acute lymphoblastic leukaemia. Essays Biochemistry, 1989; 24: 1-40.
  • 13. Hold JG: Facultative anaerobic gram-negative rad. In: Bergey's manual of Determinative Bacteriology. 9th Edn.; Williams & Wilkins, Baltimore, 1994:99179
  • 14. Kafkewitz D, Goodman D: L-asparaginase Production by the Rumen Anaerobe Vibrio succinogenes. Applied & Environmental Microbiology, 1974; 27(1): 206-209.
  • 15. Luning PA, Devlieghere F, Verhe R: Safety in the Agrifood Chain. Wageningen Academic Publisher, 2006; pp195.
  • 16. Manna, S, Sinha A, Sadhukhan R, Chakrabarty SL: Purification, characterization and antitumor activity of L-asparaginase isolated from Pseudomonas stutzeri MB- 405. Current Microbiology, 1995;30(5):291–298
  • 17. Mashburn LT, Wriston JC(Jr.): Tumor Inhibitory Effect of L-asparaginase from Escherichia coli. Archives of Biochemistry and Biophysics, 1964; 105:450-452.
  • 18. Mukherjee J, Majumadar S, Scheper T: Studies on nutritional and oxygen requirements for production of L-asparaginase by Enterobacter aerogenes. Applied Microbiology and Biotechnnology, 2000; 53(2): 180- 184
  • 19. Narayana KJP, Kumar KG, Vijayalakshmi M: Lasparaginase production by Streptomyces albidoflavus. Indian Journal of Microbiology, 2007; 48(3): 331-336.
  • 20. Nawaz MS, Zhang D, Khan AA, Cerniglia CE: Isolation and characterization of Enterobacter cloacae capable of metabolizing asparagine. Applied Microbiology & Biotechnology, 1998; 50(5): 568-572.
  • 21. Oettgen HF, Old LJ, Boyse EA, Campbel HA, Philips FS, Clarkson BD, Tallol L, Leeper RD, Schiwartz MK, Kim JH: Inhibition of leukemias in man by Lasparaginase. Cancer Research, 1967;27(12):2619-2
  • 22. Olempska-Beer ZS, Merker RI, Ditto MD, DiNovi MJ: Food-processing enzymes from recombinant microorganisms – a review. Regulatory Toxicology and Pharmacology, 2006; 45:144-158
  • 23. Pattnaik S, Kabi R, Janaki RK, Bhanot KK: L asparaginase activity in Aeromonas sp. isolated from freshwater mussel. Indian Journal of Experimental Biology, 2000; 38(1): 1143- 1146
  • 24. Peterson RE, Ciegler A: L-asparaginase production by various bacteria. Applied Microbiology, 1969; 17(6):929- 930
  • 25. Sarquis MIdeMoura, Oliveira EMM, Santos AS, da Costa GL: Production of L-asparaginase by filamentous fungi. Memórias do Instituto Oswaldo Cruz, 2004; 99(5): 489-492
  • 26. Savitri, Asthana N, Azmi W: Microbial L-Asparaginase: A potent antitumor enzyme. Indian Journal of Biotechnology, 2003; 2:184-194.
  • 27. Schwartz JH, Reeves JY, Broome JD: Two Lasparaginases from E. coli and their action against tumors. Proceedings of the National Academy of Sciences, U.S.A., 1966;56(5):1516-1519.
  • 28. Schwartz JH: An effect of streptomycin on the biosyn-thesis of the coat protein of Coliphage f2 by extracts of E. coli. Proceedings of the National Academy of Sciences, U.S.A., 1965; 53(5):1133-1140
  • 29. Sieciechowicz KA, Ireland RJ: Isolation and properties of an asparaginase from leaves of Pisum sativum. Phytochemistry, 1989; 28(9): 2275-2279.
  • 30. Story MD, Voehringer DW, Stephens LC, Meyn RE : Lasparaginase kills lymphoma cells by apoptosis. Cancer Chemotherapy and Pharmacology, 1993; 32(2):129-133.
  • 31. Swain Al, Jaskolski M, Housset D, Rao JK, Wlodawer A: Crystal structure of Escherichia coli L-asparaginase, an enzyme used in cancer therapy. Proceedings of the National Academy of Sciences U.S.A., 1993; 90(4): 1474–1478.
  • 32. Tosa T, Sano R, Yamamoto K, Nakamura M, Ando K, Chibata I: L-Asparaginase from Proteus vulgaris. Applied & Environmental Microbiology, 1971; 22(3): 387-392.
  • 33. Waksman SA, Reilly HC: Agar-streak method for assaying antibiotic substances. Analytical Chemistry, 1945; 17(9): 556-558.