Antidiabetic effects of durian (Durio zibethinus, Family: Bombacacae) leaves on alloxan-induced diabetic Sprague Dawly rats

This study aimed to determine the anti-diabetic effects of durian leaves on alloxan-induced diabetic Sprague Dawley rats. About five (5) kilograms of fresh leaves of Durio zibethinus: Bombacaceae was obtained in Silang, Cavite, Philippines. The fruit leaves were obtained and washed with fresh water and damaged parts were removed. The plant samples were washed thoroughly with distilled water, pressed, air-dried, and were taken to the Bureau of Plant Industry of the Department of Agriculture of the Philippines in Manila for authentication. Alloxan monohydrate (Sigma Chemical Company, USA) was used to induce diabetes in mice and Glibenclamide was used as a standard hypoglycemic drug. Ethanol and distilled water were used for extraction of the plant materials. ACCU CHEK Performa Glucometer was used to measure the blood glucose level. The following chemicals were used for phytochemical screening test: Chloroform and ethyl acetate, hydrochloric acid, ferric sulfate, lead acetate and potassium ferrocyanide, petroleum ether 60-80°C, sulphuric acid, acetic anhydride and methanol and ferric. All the chemicals are of analytical grades. A total of sixteen (16) Sprague Dawley rats were utilized in the study. There were four groups in the study namely; No Treatment, Distilled Water as Negative Control, Glibenclamide as Positive Control, and Durian Extract. Rats were induced using Alloxan. Results show that the absence of a treatment will not reduce glucose level. The glucose level of Glibenclamide is significantly lower than distilled water and has no treatment. The glucose level of Durian is significantly lower than no treatment and negative control. Results also show that there is a significant decrease in the glucose level from post-alloxan to post treatment of durian. It also shows that the glucose level positive control is not significantly different from the glucose level of durian extract.