People's Journal of Scientific Research
Published June 1, 2015 | Version v1
Journal article Open

Application of AgNOR (Argyrophilic Nucleolar Organizer Regions) Staining in Distinction of Non Neoplastic and Neoplastic Endometrial Lesions

Authors/Creators

  • 1. Department of Pathology, People's College Medical Sciences & Research Centre, Bhopal,
  • 2. Department of Pathology, Chirayu Medical College & Hospital, Bhopal

Description

Study of AgNOR has been identified as a reliable indicator of cell proliferation and in turn, allows a clear distinction between benign, premalignant, and malignant epithelial changes. The aim of this study was to analyze and evaluate the application of AgNOR method as diagnostic tool in non-neoplastic and neoplastic lesions of endometrium. The study included evaluation of AgNOR staining on a total of 50 cases of endometrial specimens obtained by hysterectomy and dilatation and curettage sent for histopathology. Paraffin blocks of the above selected cases were taken for hematoxylin and eosin, and AgNOR staining. Out of total cases of endometrial specimens studied, sixteen cases were normal proliferative and secretory endometrium served as control. Twenty nine cases were non-neoplastic lesions and five cases were neoplastic. In present study, 8 cases of proliferative phase endometrium showed mean AgNOR count being 2.35 and 8 cases of secretory endometrium showed mean AgNOR count being 1.55 AgNOR/nucleus. In non-neoplastic lesion group, simple hyperplasia, complex hyperplasia and complex hyperplasia with atypia showed mean AgNOR count being 3.41, 3.71 and 4.3 AgNOR/nucleus respectively. In neoplastic lesions benign endometrial polyp showed mean AgNOR count 3.48 AgNOR/ nucleus, whereas cases of endometrial carcinoma showed mean AgNOR count 5.62 AgNOR/nucleus. ANOVA t test was done and all the values were found to be statistically significant (p value< 0.0001). Present study suggests that AgNOR counts are reliable markers of endometrial proliferation and allow a clear distinction between non-neoplastic and neoplastic lesions of endometrium

Files

4 Dr Tanvi Aggarwal.pdf

Files (205.1 kB)

Name Size Download all
md5:41ef6bd87408777935b439018e8d581b
205.1 kB Preview Download

Additional details

References

  • 1. Chalise S, Thapa S, Sayami G, Shrestha A. Argyrophilic nucleolar organizer regions of thyroid lesions on fine needle aspiration smear. J Pathol Nep 2013; 3: 361-66.
  • 2. Kaushik R, Sudarshan S, Vinay M. Agnors in endometrial lesions. Indian J Pathol 1999;42(4):451-54.
  • 3. Mukhopadhyay S. Quantitative estimation of AgNORs in inflammatory gingival overgrowth in paediatric patients and its correlation with the dental plaque status. Journal of Indian Society of Pedodontics and Preventive Dentistry 2009; 27(4): 235-41.
  • 4. Derenzini M, Trere D, Oliveri F, David E, Colombatto P, Bonino F, et al. Is high AgNOR quantity in hepatocytes associated with increased risk of hepatocellular carcinoma in chronic liver disease? J Clin Pathol 1993;46:727-29.
  • 5. Dr. Sushil Lakra. AgNOR expression in Central Nervous System Tumours. J Bio Med Scien 2011;4(1):1-9.
  • 6. Nikicicz EP, Norback DH. Argyrophilic nucleolar organiser region (AgNOR) staining in normal bone marrow cells. J Clin Pathol 1990;43:723-27.
  • 7. Ahmadi SA, Samadi N. Evaluation of argyrophilic nucleolar organizer region staining in predicting the behavior of meningiomas. Ann Saudi Med 2006;26(1):38-2.
  • 8. Crocker J, Skilbeck N. Nucleolar organizer region associated proteins in cutaneous melanotic lesions: a quantitative study. J Clin Pathol 1987;40:885-89
  • 9. Terlikowski S, Lenczewski A, Famulski W, Sulkowski S, Kulikowski M. Expression of nucleolar organizer regions (NORs) in ovarian epithelial tumors. Folia Histochem Cytobiol. 2001;39(2):161-62
  • 10. Darne JF, Polacarz SV, Sheridan E, Anderson D, Ginsberg RF. Sharp Nucleolar organiser regions in adenocar-cinoma in situ and invasive adenocarcinoma of the cervix. J Clin Pathol 1990;43(8):657-60.
  • 11. Böcker W, Decker T, Ruhnke M, Schneider W. Pathologe AgNOR analysis of atypical ductal hyperplasia and intraductal carcinoma of the breast. Jpn J Cancer Res 1997;18(1):3-18.
  • 12. Lipponen PK, Eskelinen MJ, Nordlings S. Nucleolar organiser regions (AgNORs) as predictors in transitional cell bladder cancer. Br J Cancer 1991;64:1139-44.
  • 13. Rodrigues OR, Antonangelo L, Yagi N, Minamoto H, Schmidt AF, Capelozzi VL et al. Prognostic Significance of Argyrophilic Nucleolar Organizer Region (AgNOR) in Resected Non-small Cell Lung Cancer (NSCLC). Jpn J Clin Oncol 1997;27(5):298-4.
  • 14. Ohki T, Akakura K, Ueda T, Akimoto S, Yatani R, Shimazak J. Changes in Histologic Grade and Argyrophilic Nucleolar Organizer Regions during Progression of Prostate Cancer. Jpn J Clin Oncol 1996;26:82-7.
  • 15. Niwa K, Yokoyama Y, Tanaka T, Mori H, Mori H, Tamaya T. Silver-stained nucleolar organizer regions in the normal, hyperplastic and neoplastic endometrium. Virchows Arch A 1991;419(6):493-97.
  • 16. Brustmann H, Riss P, Naudé S. Nucleolar organizer regions as markers of endometrial proliferation: A study of normal, hyperplastic, and neoplastic tissue. Human Pathol 1995;26(6):664-67