Design of a redox-proficient Escherichia coli for screening terpenoids and modifying cytochrome P450s

20 Terpenoid scaffold.zip: Raw GCMS data for titer comparisons. Required to reproduce Figure 2b.

20 Terpenoid scaffold_representative GCMS.zip: Representative GCMS data for 20 terpenoid scaffolds produced by E. coli MEV15 and 20. Required to reproduce Supplementary Figure 3.

Characterization of LRD04 derivatives.zip: Raw NMR and GCMS data for 19 ent-kaurenoid derivatives described in this manuscript. Required to reproduce Supplementary Figures 13-33.

LRD production optimization.zip: Raw GCMS data for pLRD construct screening with different IPTG concentration. Required to reproduce Supplementary Tables 9-36.

LRD scaffolds.zip: Raw GCMS data for LRD production after optimization. Required to reproduce Figure 5 and Supplementary Tables 9-36.

Pathway screening.zip: Raw GCMS data for screening biosynthetic pathways of LRD scaffold paired with 64 CYPs. Each GCMS dataset is consisted of 1 pathway producing only LRD scaffold, 64 pathways producing LRD scaffold and different CYPs, and 2 alkane-series standards acquired before and after analyzing the 65 pathways. Required to reproduce Figure 6 and Supplementary Tables 9-36.

Redox array characterization 1.zip: Raw GCMS data for comparison of modified terpenoid production in the presence of different redox enzymes. Required to reproduce Figure 3a.

Redox array characterization 1_scaffold.zip: Raw GCMS data for comparison of terpenoid scaffold production in the presence of different redox enzymes. Required to reproduce Supplementary Figure 4.

Redox array characterization 2.zip: Raw GCMS data for comparison of modified terpenoid production with or without an additional copy of fldA/fpr. Required to reproduce Figure 3b and Supplementary Figure 5.

Terpenoid inducer optimization.zip: Raw LCMS and GCMS data for optimizing 01a and 03a production in E. coli MEV20. Required to reproduce Figure 4 and Supplementary Figures 6 and 7.