Fig. 7. Compound 14 reduced LPS-induced inflammatory factors production and inhibited NF-κB nuclear translocation. (A–C) The expressions of iNOS and COX-2 were determined by Western blot analysis. (D–F) The expressions of NF-κB, p-IκBα, and IκBα were determined by Western blot analysis. The quantification of protein expression was performed by Image J. (G) The inhibitory effect of compound 14 (16 μM) on the nuclear translocation of NF-κB p65 induced by LPS via confocal laser scanning microscopy. RAW264.7 cells stained for NF-κB (green) and nuclei (DAPI, blue) (scale bar: 10 μm). The values were presented as mean ± SD of three independent experiments, n 3. ###p <0.001, vs. the control group; **p <0.01, ***p <0.001, vs. LPS-treated group. (For interpretation of the references to color in = this figure legend, the reader is referred to the Web version of this article.)