Published August 2, 2023
| Version v1
Dataset
Open
CRISPR-based engineering of RNA viruses
Description
CRISPR RNA-guided endonucleases have enabled precise editing of DNA. However, options for editing RNA remain limited. Here, we combine sequence-specific RNA cleavage by CRISPR ribonucleases with programmable RNA repair to make precise deletions and insertions in RNA. This work establishes a new recombinant RNA technology with immediate applications for the facile engineering of RNA viruses.
This dataset contains code for analyzing sequencing data and generating figures in the manuscript.
Notes
Link to the preprint https://doi.org/10.1101/2023.05.19.541219
Files
Additional details
Related works
- Is supplement to
- Dataset: https://dataview.ncbi.nlm.nih.gov/object/PRJNA998050 (URL)
Funding
- National Institutes of Health
- Structural and functional understanding of bacterial defense and viral counter defense 1R35GM134867-01
- National Institutes of Health
- CRISPR-based editing of RNA and cellular mechanisms that govern RNA repair 1K99AI171893-01