Macromolecular Crystallography
Published June 19, 2017 | Version v1
Dataset Open

Diffraction images of a crystal of the spectrin repeats 7, 8, and 9 (SR7-SR9) of the plakin domain of human plectin (PDB code 5J1I)

Creators

  • 1. Instituto de Biología Molecular y Celular del Cancer, Consejo Superior de Investigaciones Científicas, University of Salamanca, 37007 Salamanca, Spain

Description

Diffraction images of crystals of a fragment of the plakin domain of human plectin that includes the spectrin repeats 7 to 9 (SR7-SR9).

Images correspond to the dataset used to solve and refine the pdb entry 5J1I (http://www.rcsb.org/pdb/explore/explore.do?structureId=5J1I).

Data were collected on a single crystal at the beamline 14.2 of the European Synchrotron Radiation Facility (ESRF, Grenoble, France) using radiation of 0.9330 Å wavelength and an ADSC Q4 CCD detector. The dataset consists of 360 images (1 degree oscillation per image).

Diffraction data is highly anisotropic. Based on analysis with the STARANISO server (http://staraniso.globalphasing.org/) data extend approximately to 5.0, 3.8, and 2.6 Å resolution along the three principal directions of anisotropy, which are 0.555 a*+ 0.832 c*, b*, and -0.361 a* + 0.932 c*, respectively.

Notes

This work was supported by the Spanish Ministry of Economy and Competitiveness (MINECO) and the European Regional Development Fund, Grants BFU2012-32847 and BFU2015-69499-P. We thank the ESRF for providing access to synchrotron radiation facility.

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Additional details

Related works

Is supplement to
10.2210/pdb5j1i/pdb (DOI)
10.1074/jbc.M116.732909 (DOI)

References

  • Ortega E, Manso JA, Buey RM, Carballido AM, Carabias A, Sonnenberg A, de Pereda JM (2016) "The Structure of the Plakin Domain of Plectin Reveals an Extended Rod-like Shape." J Biol Chem 291(36), 18643-18662.