Figure 1. Nav1.5 co-immunoprecipitated with 14-3-3η.

Fig 1. Nav1.5 co-immunoprecipitated with 14-3-3η. (a) Immunoblot of total lysate and Nav1.5-specific immunoprecipitated fraction of tsA201 cells 48 hours after transient co-expression of an empty vector, 14-3-3σ, 14-3-3η and Nav1.5. Immunoprecipitation was performed with sepharose beads pre-coupled with anti-Nav1.5 antibody. (b) Immunoblot of the control experiment testing aspecific binding of 14-3-3η to sepharose beads pre-coupled with immunoglobulin G (IgG) and anti-Nav1.5 antibody. HA-tagged 14-3-3σ and 14-3-3η were revealed with an anti-HA antibody. Endogenous α1-syntrophin was used as a positive control for co-immunoprecipitation with Nav1.5, and α-actin as a negative control. Yellow triangle indicates on the band corresponding to the co-immunoprecipitated 14-3-3η.