Supplemental material for the paper "PCR-based strategy for introducing CRISPR/Cas9 machinery into hematopoietic cell lines"

Supplemental Table S1: Results of NHEJ obtained with ICE analysis with different optimizations in HEK293 cells; Supplemental Table S2: Results of NHEJ obtained with ICE analysis with different sgIDH2 in HEK293 cells; Supplemental Table S3: NHEJ efficiencies obtained with ICE anlysis in NB4-Cas9 cells using sgIDH2 constructs or NB4 cells using RNPs; Supplemental Table S4: sgRNA and ssODN sequences; Supplemental Table S5: Primers sequence for U6-sgRNA-terminator cassette amplification in PX458 vector; Supplemental Table S6: Primers sequence for sgRNA constructs; Supplemental Table S7: Primers used in PCR 1 for library preparation; Supplemental Table S8: Primers used in PCR 2 for library preparation; Supplemental Table S9: Primers used to amplify Cas9 cassette from hCas9 vector; Supplemental Table S10: Primers used to amplify targeted region in MYBL2 and IDH2 for T7 and RFLP assays and ICE analysis; Supplemental Table S11: Primers used to detect Cas9 lentiCRISPR V2 integration in cell lines.