Published February 9, 2022 | Version 1.0.0

Data set for the journal article: Site-Specific Protein Ubiquitylation Using an Engineered, Chimeric E1 Activating Enzyme and E2 SUMO Conjugating Enzyme Ubc9

  • 1. Department of Chemistry and Applied Biosciences, ETH Zürich, 8093 Zürich, Switzerland

Description

Mutations observed in evolved chimeric E1 variants. Top row (1.X to 4.X) describes rounds of evolutions with respective variants in the round. 

Residues that appear to be enriched are highlighted with gray fill. Star (★) marks residues subjected to saturation mutagenesis in the round 4.

Files

Files (17.8 kB)

Name Size Download all
md5:92ea080add4223de7272bb5bf422c1ec
17.8 kB Download

Additional details

Related works

Is supplement to
Journal article: 10.1021/acscentsci.1c01490 (DOI)

Funding

Swiss National Science Foundation
NCCR Catalysis (phase I) 51NF40_180544