Molecular Analysis of Resistance Genes to Brown Rust Lr9, Lr19, Lr34, Lr35 and Yellow Rust Yr9, Yr18 in Azerbaijan Wheat Germplasm

Rust is the most devastating fungal disease causing significant losses of grain production. Growing rust
resistant varieties is the most sustainable, cost-effective and environmentally friendly approach for
controlling rust diseases. To date, 81 brown and 53 yellow rust resistance genes have been detected in
bread and durum wheat genotypes and their wild species using different molecular methods. The pri-
mary goal of the research was to establish the presence of Lr ans Yr genes in wheat t samples collected in
Gene Pool of the Reserch Institute of Crop Husbandry (Baku, Azerbaijan). Four Lr genes, Lr9, Lr19,
Lr34, Lr35 and two Yr genes, Yr9 u Yr18 were analysed using various molecular markers. 1100 bp spe-
cific fragments in PCR profiles indicate the presence of Lr9 gene on 6B chromosome of 60% of the
studied 78 genotypes. Positive results were obtained in 45 genotypes for identification of Lr19 gene us-
ing SCAR markers SCS123 and SCS253. Allelic state of Lr34 gene was studied using Lr34/csLV34a
and Lr34/csLV34b markers. Molecular analysis showed the presence of allele a of Lr34 in 21 and allele
b in 9 genotypes. Two genotypes were found to carry both alleles of Lr34. 54% the studied wheat
genotypes had no allele of Lr34 gene. Fragments characteristic of Lr35 gene were not visualized in
PCR profiles of 61 genotypes. Fragments of 250 bp diagnostic for Yr18 gene were visualized in electro-
phoretic profiles of 40 genotypes. 150 bp fragments characteristic of Yr9 gene were amplified in all
genotypes with the exception of four samples. These results will serve as a base for plant breeders to
develop durable rust-resistant wheat varieties and to control wheat leaf rust diseases in Azerbaijan.