Published June 26, 2023 | Version v1
Dataset Open

Datasets underlying the paper Zero-mode waveguide nanowells for single-molecule detection in living cells

Authors/Creators

  • 1. Oncode Institute, Hubrecht Institute–KNAW and University Medical Center Utrecht, Utrecht, the Netherlands
  • 2. Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, The Netherlands

Contributors

  • 1. Oncode Institute, Hubrecht Institute–KNAW and University Medical Center Utrecht, Utrecht, the Netherlands
  • 2. Delft University of Technology

Description

Different datasets underlying the paper Zero-mode waveguide nanowells for single-molecule detection in living cells. The repository contains .zip archives, mostly containing a readme file with additional information.

Cell imaging experiments.zip contains the raw image files acquired on arrays of version 1 or version 2 using a Nikon TI inverted microscope and used in figures 4-6.

Gla_0127_14.zip contains SEM images of the fabrication of arrays of version 1

Gla_29_Pd_1.zip contains SEM images of the fabrication of arrays of version 2

SM experiments.zip contains the raw single-molecule fluorescence data acquired on an array of version 1 using a PicoQuant Microtime microscope together with the analysis files.

FDTD simulations.zip contains the simulation files for the use in the software Lumerical

Notes

Additional funding: NWO program OCENW.GROOT.2019.068, NanoFront and BaSyC programs of NWO/OCW.

Files

Cell imaging experiments.zip

Files (5.3 GB)

Name Size
md5:983f1dc85584d0f4a3b660f8c17cafac
1.2 GB Preview Download
md5:3faf6feb54090dffe5c617e1eaadeddd
1.6 GB Preview Download
md5:683bf3e8201b0a57354e96eb9642a9ed
24.1 MB Preview Download
md5:7881fcd2b0fc84e093a49b3f1bfd3444
27.0 MB Preview Download
md5:248995581f5b8f5ab8759cea40236de2
2.6 GB Preview Download

Additional details

Related works

Is published in
Preprint: 10.1101/2023.06.26.546504 (DOI)

Funding

European Commission
LoopingDNA - Bottom up biophysics approach to resolve the looping structure of chromosomes 883684
European Commission
NTON - Unravelling the mechanism of nuclear transport using optical nanopores 101029907
European Commission
smTI - Visualisation of translation initiation reaction on single mRNA molecules in vivo 101026470