Whole-genome capture and sequencing of Francisella tularensis directly from clinical samples

This dataset comprises:

1. The design of RNA oligonucleotide baits for Agilent Technologies’ SureSelect target enrichment - 54756 RNA oligonucleotide "baits" (120 bp each) designed to perform whole-genome capture and sequencing of Francisella tularensis directly from clinical samples (DNA) using Agilent Technologies’ SureSelect target enrichment system following the Illumina paired-end multiplexed sequencing library protocol.

RNA oligonucleotide “baits” were designed to span the Francisella tularensis chromosome and plasmid, accounting for the genetic variability among publicly available genome sequences. All resulting sequences were BLASTn searched against the Human Genomic + Transcript database to excluded homologous sequences to the human genome. Overall, a total of 54756 RNA probes were generated and this custom bait library was then uploaded to the SureDesign software (https://earray.chem.agilent.com/suredesign) and synthesized by Agilent Technologies.

2. Genome assemblies of 17 Francisella tularensis samples generated in the context of validation and application of SureSelect target enrichment for Whole-genome capture and sequencing of Francisella tularensis directly from clinical samples  

File “Ft_assembly_metadata.xlsx” contains the genome assembly statistics for each isolate, including European Nucleotide Archive accession numbers.

The archive “Ft_assemblies.zip” contains all the genome assemblies (.fasta format) of each isolate presented in the metadata file.

More details can be found in the following publication: (available soon)