Published June 12, 2023
| Version Version 1
Dataset
Open
snATAC-seq and snRNA-seq in the chromatin landscape of healthy and injured cell types in the human kidney
Authors/Creators
- 1. Washington University in Saint Louis School of Medicine: Saint Louis, MO, US
- 2. Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA
- 3. Indiana University School of Medicine, Indianapolis, IN, 46202, USA
Description
Isolated nuclei were immediately processed using the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression (v1.0) kit, following the step-by-step protocol provided online [https://dx.doi.org/10.17504/protocols.io.5qpvoby69l4o/v2].
The RNA and ATAC libraries were separately sequenced on the NovaSeq 6000 system (Illumina) using the NovaSeq Control Software versions 1.7.0 and 1.7.5. Sample demultiplexing, barcode processing, gene expression quantification, and open chromatin peak quantification were performed using the Cell Ranger Arc software (version 2.0.0) with the GRCh38 (hg38) reference genome.
Published in: DOI: 10.1038/s41467-023-44467-6
Files
KPMP_20210421E_10X_Dual.zip
Files
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Additional details
Dates
- Available
-
2024-02-13