Published June 8, 2023
| Version v1
Dataset
Open
Spatial Mapping and Host Linking of Mobile Genetic Elements in Complex Microbiomes - Combined taxonomic mapping and MGE mapping
Authors/Creators
- 1. Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA
Description
We used rRNA FISH to stain five common oral genera, Veillonella, Streptococcus, Corynebacterium, Lautropia, and Neisseria, each with a different fluorophore, and we used MGE-FISH to stain the termL gene of an active prophage with a sixth fluorophore.
We chose a target panel of 18 genera that are highly abundant and prevalent in human plaque and designed a HiPR-FISH probe panel using a 5-fluorophore combinatorial barcoding scheme. Using MGE-FISH, we stained a plasmid carrying mefE, subunit of a major-facilitator-superfamily antibiotic efflux pump.
Files
Files
(11.0 GB)
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