IN-VITRO EVALUATION OF ANTI-MITOTIC ACTIVITY OF DIFFERENT EXTRACTS OF ABUTILON INDICUM Linn. SEED

Abutilon indicum is a medicinal plant with therapeutic potential, belongs to the familymalvacea, also known as Duvvena Kayalu, and is known for its anti-mitotic activity. Recently, many biological activities of Abutilon indicumL. Seeds have been reported, including antioxidant, anti-cholesterol, anti-Parkinson, antidiabetic, sexually enhancing, anti-inflammatory, antimicrobial, anti-cancer, and antivenom activities. In this study, several seed extracts from Abutilon indicumL. Have been tested for Antimitotic activity. The assay results concluded that the ethanolic seed coat extract and ether seed coat extract of Abutilon indicum showed inhibition of green gram seed growth of 7.2±4.6 mm and ether seed coat extract showed inhibition of 0.01±1.5mm with a concentration of20mg/ml after 24hrs, 48hrs and 72hrs, compared with percentage inhibition of standard Methotrexate of 0.001±1.1mm with a concentration of 0.1. Among all the seed extracts, ether extract showed more anti-mitotic activity compared with ethanolic extract. In the point of view of seed germination the control, i.e., distilled water, has shown higher seed germination compared to standard and other ethanolic and ether seed coat extracts.

Key words: In-vitroAnti-mitotic activity, Abutilon indicum, L. seed. Methotrexate, Green Gram