REVIEW ON QUALITATIVE AND QUANTITATIVE ANALYSIS OF DRUGS BY UV-VISIBLE SPECTROPHOTOMETER

One of the earliest instrumental techniques for analysis is UV-VIS spectroscopy. Many different types of materials can be characterised using UV-VIS spectroscopy. The UV-VIS delivers data based on the degree of absorption or transmittance in a varied wavelength of light and responses of sample. The fundamental principle of operation UV-VIS spectrophotometer is Beer’s- Lambert’s law. The UV-VIS spectrophotometer is simple to use and handle and also less expensive. The analysis of sample is done at the wavelengths between 200nm-700nm. This review’s goal is to provide information on the principle, instrumentation and mainly provides information regarding the methodology used for the qualitative and quantitative analysis of drugs by using UV-VIS spectroscopy.  The pharmaceutical analysis comprises the procedure necessary to determine the “identity, strength, quality and purity” of such compounds. It also includes the analysis of raw materials and intermediates. This method is quick, simple and affordable. Here is a quick overview of the theories, instrumentation and methodology used for the qualitative and quantitative analysis of drug samples using UV-VIS spectroscopy.

Keywords: UV-VIS spectroscopy, Spectroscopy, Analysis of drugs.