Proteomics analysis for: The platelet transcriptome and proteome in Alzheimer's disease and aging: an exploratory cross-sectional study
- Bessa de Sousa, Diana M1
- Poupardin, Rodolphe2
- Villeda, Saul3
- Schroer, Adam3
- Fröhlich, Thomas4
- Frey, Vanessa5
- Staffen, Wolfgang5
- Mrowetz, Heike6
- Altendorfer, Barbara7
- Unger, Michael S8
- Iglseder, Bernhard5
- Paulweber, Bernhard9
- Eugen, Trinka10
- Cadamuro, Janne11
- Drerup, Martin12
- Schallmoser, Katharina13
- Aigner, Ludwig14
- Kniewallner, Kathrin M15
- 1. Institute of Molecular Regenerative, Institute of Molecular Regenerative Medicine, Paracelsus Medical University, Salzburg, Austria Medicine, Paracelsus Medical University, Salzburg, Austria, Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University, Salzburg, Austria
- 2. Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University, Salzburg, Austria , Experimental and Clinical Cell Therapy Institute, Paracelsus Medical University, Salzburg, Austria
- 3. Department of Anatomy, University of California, San Francisco, California, United States of America
- 4. Laboratory of Functional Genome Analysis (LAFUGA), Gene Center, Ludwig Maximilian University of Munich, Munich, Germany
- 5. Department of Neurology, Christian Doppler Clinic, Paracelsus Medical University, Salzburg, Austria
- 6. Institute of Molecular Regenerative, Medicine, Paracelsus Medical University, Salzburg, Austria, Institute of Molecular Regenerative Medicine, Paracelsus Medical University, Salzburg, Austria, Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University, Salzburg, Austria
- 7. Institute of Molecular Regenerative Medicine, Paracelsus Medical University, Salzburg, Austria, Institute of Molecular Regenerative Medicine, Paracelsus Medical University, Salzburg, Austria , Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University, Salzburg, Austria
- 8. Institute of Molecular Regenerative Medicine, Paracelsus Medical University, Salzburg, Austria, Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University, Salzburg, Austria
- 9. Department of Internal Medicine, St. Johanns University Hospital, Paracelsus Medical University, Salzburg, Austria
- 10. Department of Neurology, Christian Doppler Clinic, Paracelsus Medical University, Salzburg, Austria, Department of Public Health, Health Services Research and Health Technology Assessment, UMIT-University for Health Sciences, Medical Informatics and Technology, Hall in Tirol, Austria, Neuroscience Institute, Christian Doppler University Hospital, Paracelsus Medical University and Centre for Cognitive Neuroscience Salzburg, Austria
- 11. Department of Laboratory Medicine, University Hospital SALK, Salzburg, Austria
- 12. Department of Urology, Paracelsus Medical University, Salzburg, Austria
- 13. Department of Transfusion Medicine, Paracelsus Medical University, Salzburg, Austria , Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University, Salzburg, Austria
- 14. Institute of Molecular Regenerative Medicine, Paracelsus Medical University, Salzburg, Austria , Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University, Salzburg, Austria , Austrian Cluster for Tissue Regeneration, Vienna, Austria
- 15. Institute of Molecular Regenerative Medicine, Paracelsus Medical University, Salzburg, Austria,Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University, Salzburg, Austria
Description
Alzheimer’s disease (AD) and aging are associated with platelet hyperactivity. However, the mechanisms underlying abnormal platelet function in AD and aging are yet poorly understood. To explore the molecular profile of AD and aged platelets, we investigated platelet activation (i.e., CD62P expression), proteome and transcriptome in AD patients, non-demented elderly, and young individuals as controls. AD, aged and young individuals showed similar levels of platelet activation based on CD62P expression. However, AD and aged individuals had a proteomic signature suggestive of increased platelet activation compared with young controls. Transcriptomic profiling suggested the dysregulation proteolytic machinery involved in the regulation of platelet function, particularly in the ubiquitin-proteasome system in AD and autophagy in aging. The functional implication of these transcriptomic alterations remains unclear and requires further investigations.
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