Anti-HIV Activity and Cytotoxic Effects of Aerva lanata Root Extracts

ABSTRACT

The aim of the present study was to evaluate the anti-HIV activity and cytotoxic effects of Aerva lanata root extracts. Extracts were
prepared in hexane, chloroform, ethyl acetate, acetone and methanol solvents by sequential maceration method and the extract was filtered mass was obtained at low room temperature under pressure in a rotary vaccum evaporator. Anti-HIV activity of the all solvent
extracts of Aerva lanata root done by Retro Sys HIV-1 RT activity kit (Innovagen, Sweden). Cytoxicity study was performed on all
extracts by MTT assay using PBMCs isolated from whole blood. All extracts exhibited the most notable activity and the chloroform
extract of Aerva lanata showing highest (91.0%) HIV-RT inhibition at 2mg/ml concentration, hexane, ethyl acetate and acetone
extractions showed highest inhibition of HIV-RT at 2 mg/ml concentration (86.9, 85.2 and 77.5 respectively). While control drug
(AZT) showing 91.7% at 2mg/ml concentration. IC50 value of all extracts determined below 40mg/ml. This result suggests that Aerva lanata root extracts contains medicinally important bioactive compounds and this justifies the use of plant species as traditional medicine for treatment of various infectious diseases.

Keywords: HIV-RT, AIDS, PBMC’s, Cytotoxicity, Aerva lanata

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