Unedited photographs of Mycobacterium tuberculosis colonies to practice an image processing protocol

Unedited raw photographs in TIFF file format of Mycobacterium tuberculosis (Mtb) colonies grown in 7H10 agar plates inside a BSL-3 facility. To assay the bactericidal effect of different drug treatments, serial dilutions were performed per treatment in plate quadrants to ensure a countable range of colony-forming units (CFU). Each treatment has three replicates and a control without the antibacterial treatment. The plates were incubated until colonies grew to about 1–2 mm in size. Photographed using a Sony Alpha 7R II full-frame mirrorless color camera (7974 x 5316 pixels) under ambient laboratory lighting at a distance of 12–18 inches from the plates after placing the plates on a black background and behind the glass of the biosafety cabinet.