Tracking cell edge movement

MATLAB scripts used to quantify cell edge movements from pixel intensity profiles (obtained in each frame of time-lapse sequences along a line that was placed across the cell of interest) generated by the Linescan feature of MetaMorph software.  

These algorithms were originally designed for the measurements of the outer hair cell electromotility with the accuracy of ~20 nm by interpolating pixel values along each line to increase the number of points (Frolenkov, G.I. et al, Biophys J, 1997).