Impact of In-Ovo inoculation of Aloe Vera (L.) Burm. F. Medicinal Plant Leaf Extracts on Pre-hatching Growth Responses of Embryo of Broiler Chickens

In-Ovo injection with exogenous materials (Plants leaf extract) used as natural growth promoters, throughout incubation could be a technique to boost up pre-hatchling performance of broiler embryo. The objective of the current study was to observe the impact of in-Ovo injection of Aloe vera leaf extracts on the subsequent pre-hatching growth responses of broiler chickens. One hundred twenty fertile eggs of ISA Brown layer breeders were used in the current study and purchased from District Poultry Farm, Dera Ghazi Khan. The eggs were randomly distributed into four groups. Group 1 (control group, without any injection), group 2 received an injection of 100 ml of 0.9% normal saline, 100 ml of 10μg/ml of Aloe vera was inoculated in eggs of group 3, group 4 was inoculated with 100 ml of 100μg/ml of Aloe vera. The plant leaf extract was injected on the 5th day of incubation and embryonic growth parameters were observed after fourteen days of incubation. A highly significant (P-0.000) result was observed in body and liver weight, body, beak, fore limb, hind limb, eyes, toes, and shank length. Kidney weight and relative weight of the liver showed significant results (P-0.04). Non-significant behavior was examined in other embryogenic attributes such as head diameter, gonads and heart weight, and relative weight of kidney, heart, and gonads According to the results, it can be concluded that In-Ovo inoculation of Aloe vera leaf extracts on the 5th day of incubation has a positive impact on embryonic growth performance.