Other Open Access

Polarised light modular microscopy (Pol-ModMicro) for identifying hemozoin crystals in Plasmodium

Fraser Eadie; Matthew P Gibbins; Graham Gibson; Matthias Marti; Akhil Kallepalli

In this repository, you will find all the files needed to recreate the Pol-ModMicro system. These include: 

  1. Unmodified OpenFlexure files; as used in the system. 
  2. Modified/Updated Components to allow ModLight integration
  3. OpenFlexure Nano Motor Controller files
  4. Bill of Materials

Abstract: Plasmodium spp. are the protozoan parasites responsible for malaria. Plasmodium spp. synthesise a biocrystal, hemozoin, which can be observed under cross-polarised light. These birefringent crystals can be seen due to different refractive indices of the hemozoin crystal and red blood cells. Here, we present a polarised light modular microscopy (Pol-ModMicro) solution, complete with illumination sources and a robust imaging system to capture birefringence and identify the parasites. We achieve this by combining ModLight light sources with bespoke components designed for the OpenFlexure microscope to image blood smears. Further, a simple and robust algorithm capable of enhancing birefringence is presented. This solution provides image quality that is comparable to a substantially more expensive proprietary microscope. 

This work was made possible with support from the Leverhulme Trust Early Career Fellowship, EPSRC Impact Acceleration Account (IAA) [EP/R511705/1] and EPSRC funding to QuantIC [EP/M01326X/1].
Files (4.7 MB)
Name Size
Pol-ModMicro.zip
md5:b5d795d5d4194fdf2214fcf8427e9f6a 		4.7 MB Download
31
0
views
downloads
All versions This version
Views 3131
Downloads 00
Data volume 0 Bytes0 Bytes
Unique views 2727
Unique downloads 00
More info on how stats are collected.
Indexed in
Publication date:
April 26, 2023
DOI:
10.5281/zenodo.7837389

Zenodo DOI Badge

DOI 

10.5281/zenodo.7837389

Markdown 

[![DOI](https://zenodo.org/badge/DOI/10.5281/zenodo.7837389.svg)](https://doi.org/10.5281/zenodo.7837389)

reStructedText 

.. image:: https://zenodo.org/badge/DOI/10.5281/zenodo.7837389.svg
   :target: https://doi.org/10.5281/zenodo.7837389

HTML 

<a href="https://doi.org/10.5281/zenodo.7837389"><img src="https://zenodo.org/badge/DOI/10.5281/zenodo.7837389.svg" alt="DOI"></a>

Image URL 

https://zenodo.org/badge/DOI/10.5281/zenodo.7837389.svg

Target URL 

https://doi.org/10.5281/zenodo.7837389

Keyword(s):
polarised light microscope polarisation malaria plasmodium open source microscope
License (for files):
Creative Commons Attribution 4.0 International

Versions

Version 1 10.5281/zenodo.7837389 Apr 26, 2023
Cite all versions? You can cite all versions by using the DOI 10.5281/zenodo.7837388. This DOI represents all versions, and will always resolve to the latest one. Read more.

Share

Cite as

Export