Published April 15, 2023 | Version v1

Systematic exploration of mitochondrial DNA- and RNA-protein complexes by complexome profiling (datasets)

  • 1. Radboud Center for Mitochondrial Medicine, Department of Paediatrics, Radboud University Medical Center, Nijmegen, The Netherlands
  • 2. Functional Proteomics, Institute for Cardiovascular Physiology, Goethe University, Frankfurt am Main, Germany

Description

Supplemental data files that include the results of complexome profiling experiments from the manuscript entitled "Let’s make it clear: Systematic exploration of mitochondrial DNA- and RNA-protein complexes by complexome profiling" by Potter et al

Supplemental Data 1: comparison of mitochondrial complexomes obtained with either Blue Native (BNE) or high resolution Clear Native (hrCNE) gel electrophoresis. 

Supplemental Data 2: comparison of mitochondrial complexomes obtained with hrCNE from samples supplemented or not with DNase I.  

Supplemental Data 3: comparison of mitochondrial complexomes obtained with hrCNE from samples supplemented with only DNase I or a combination of DNase I and RNase A.  

Supplemental Data 4: comparison of mitochondrial complexomes obtained with hrCNE from control cells, cells treated with ethidium bromide and cells recovering after ethidium bromide treatment.

Supplemental Data 5: results of LFQ-based analysis of profiles obtained from samples supplemented or not with DNase I.

Supplemental Data 6: individual profiles (replicates), alignment and mass calibration results for the presented data.

Files

Files (140.4 MB)

Name Size Download all
md5:7138d2c9213cfad00f7ba23df9a303dc
34.1 MB Download
md5:bb6d7df1898a1e2635daa6edc7a710e0
13.2 MB Download
md5:1a88e50479881a643a45aa767d0aa573
13.0 MB Download
md5:6393864f5fb9de554e8dd730fcb379dc
16.8 MB Download
md5:58a07ff656e8d3054bddaa1c53474459
2.2 MB Download
md5:27fae4091d03d484a6de3bcc389f92db
61.1 MB Download