Oncogenic signalling is coupled to colorectal cancer cell differentiation state

Mass cytometry and single-cell RNA-sequencing data as well as R Markdown reports to reproduce the figures of our publication.

Raw MC data were saved post de-convolution, spillover-compensation, and removal of calibration bead events. Gates for singlets and non-dead cells (low_Pt) are included as logical columns and should be applied prior to usage.

As we performed random sampling to equalise cell numbers across conditions, batch normalisation, and used non-linear dimensionality reduction techniques (UMAP and Diffusion Maps), resulting plots may differ slightly from the published figures, yet still support the drawn conclusions. Already normalised and/or sampled data as well as pre-computed UMAP and Diffusion Map coordinates are included in this data set to reproduce the manuscript figures exactly, as shown in the included report “figures_only”. For all details on the batch normalisation and data analysis steps performed, please consult the report “data_analysis” instead.