Study on the Influence of AI-2/LuxS Quorum Sensing System on the Biofilm Formation of Lactic Acid Bacteria
Authors/Creators
- 1. Nanyue College of Hengyang Normal University, Hengyang, 421008, P.R. China
- 2. Nanyue College of Hengyang Normal University, Hengyang, 421008, P.R. China. Hunan Key Laboratory for Conservation and Utilization of Biological Resources in the Nanyue Mountainous Region, Hengyang, 421008, P.R. China College of Life Sciences, Hengyang Normal University, Hengyang, 421008, P.R. China
- 3. Hunan Key Laboratory for Conservation and Utilization of Biological Resources in the Nanyue Mountainous Region, Hengyang, 421008, P.R. China
Description
Lactic acid bacteria (LAB) are important probiotic microorganisms used in food fermentation industry. AI-2/ LuxS is an important quorum sensing system which affects the growth, biofilm formation, virulence, and metabolism of bacteria. LuxS is encoded by the luxS gene, but how AI-2/LuxS is associated with a diverse array of physiological activities in lactic acid bacteria is not known. In this study, four strains of lactic acid bacteria S1-4, among which S1-3 were obtained from Chinese traditional fermented pickles and S4 was the luxS gene knockout mutant from the wild strain S1, were used to explore the effect of AI-2/luxS system on the biofilm formation of lactic acid bacteria. The results showed that the strains of S1-3 all produced AI-2 signal molecules except S4, but the biofilm formation ability of S4 was the highest among the 4 strains, which demonstrated that the deletion of the luxS gene upregulated the biofilm formation ability of S4. Similar results were proven by microscopic observation. It can be seen that the AI-2/LuxS system has a significant impact on biofilm formation. This study will provide the theoretical basis for further research and for the high value of utilization of lactic acid bacteria.