Published March 29, 2023
| Version 1.0.0
Dataset
Open
Clonal heterogeneity of endocrine therapy resistance in breast cancer
Authors/Creators
- 1. German Cancer Research Center (DKFZ)-Division of Molecular Genome Analysis
- 2. German Cancer Research Center (DKFZ)-Division of Somatic Evolution and Early Detection
- 3. Weizmann Institute of Science-Department of Immunology and Regenerative Biology
- 4. German Cancer Research Center (DKFZ)-Mass Spectrometry Core Facility
- 5. German Cancer Research Center (DKFZ)-Cellular Tools Core Facility
Description
We barcoded endocrine therapy sensitive cell lines (MCF7 and T47D) and rendered them resistant to commonly applied first line endocrine therapeutics (Tamoxifen and estrogen deprivation). Next, we isolated single cell clones of endocrine therapy resistant populations and subjected clonal cell lines to RNA-Seq and Phosphoproteomics profiling.
Notes
Supplementary data and material necessary for reproducing the analysis of the relative publication. The raw data of this study have been uploaded in the EGA European Genome-Phenome Archive (EGAS00001007123). Short description of the included data files:
Integration site analysis: Analysis of viral integration sites as described in the paper performed by Genewerk GmbH for selected barcoded cell line pools.
MCF7_TAMR/LTED_MiSeq_all_barcodes_gr10: Individual MCF7 TAMR and LTED clones were characterized by many barcodes. Therefore, the barcodes of individual clones were deeply sequenced on a MiSeq as described in the paper.
All_barcodes_seqq_table: contains information about all barcodes and their reads in all barcoded cell lines as described in the paper.
Files
Supplementary File 2_all_barcodes_seqq_table_MCF7_labelled.txt
Files
(381.0 MB)
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