Published March 25, 2023 | Version v1
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Method Development for Detection of 2-Methylpyridine by High Performance Liquid Chromatography

Authors/Creators

  • 1. Indian Institute of Engineering Science and Technology, Shibpur

Description

A method for detection and quantification of 2-methylpyridine (2Mp) from aqueous solution was developed in this study by utilizing High Performance Liquid Chromatography (HPLC). Reverse phase analysis was performed using a C18 column. The effect of chromatographic factors such as initial 2Mp concentration, C0 (mg/L), Acetonitrile (ACN) percent in water as carrier, and flow rate, Q (mL/min) on peak asymmetry were determined by means of mathematical modelling using three factor Doehlert design of experiments. A polynomial model equation containing constant, linear, squared and two factor interaction terms was developed for predicting peak asymmetry. The optimum values for the factors were found to be 2Mp concentration of 12.28 mg/L, ACN percent of 59 and flow rate of 0.74 mL/min. At these factor values, the model predicted an optimal peak asymmetry of 1.048, which was validated by experimental results. The results of the study were further represented by means of Response Surface Method (RSM). The most important factor affecting peak asymmetry was ACN percent. Good correlation between the experimental and model predicted data was obtained with a correlation coefficient (R2) value of 0.982. Also, the model was designed with a low prediction error of 2.2 %. Additionally, the method developed in this study was reliable and reproducible.

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References

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