Naturally segregating variants contributing to thermal tolerance in a D. melanogaster model system.

Main_Incapacitation.zip and Incapacitation_founders.zip contain raw thermal tolerance scores for individuals measured within the heat box. Each folder is labeled with the RIL or founder ID and replicates within each file are labeled with group numbers.  

RNAi_files_to_tar.txt contains the metadata for the Combined_tracks_RNAi_1.Rds.zip and  Combined_tracks_RNAi_2.Rds.zip.

Combined_tracks_RNAi_1.Rds.zip and Combined_tracks_RNAi_2.Rds.zip. contains raw data for RNAi lines measured on the heat plate. 

plate_finder-kinglab-2021-05-02.zip contains the DeepLabCut model used for finding the corners of aluminum mounting plate used to hold the fly vials for thermal sensitivity testing. This directory contains the training data as well as the trained and evaluated model. No retraining should be necessary for use.

fly_tracker_2-king-2021-09-27.zip contains the DeepLabCut model used for tracking individual flies during thermal sensitivity testing. This directory contains the training data as well as the trained and evaluated model. No retraining should be necessary for use.

fly_tracker_batch.py is a python (>= 3.0) script that processes the raw movie files collected via the Raspberry Pi. This script uses the plate finder DeepLabCut model to find the corners of the plate, rotate and crop the images, and output movie files for individual flies. It then uses the fly tracker DeepLabCut model to track the flies and output the data for subsequent processing in R.