Published March 24, 2023 | Version v1

Multiplexed On-Yeast Serological Assay for Immune Escape Screening of SARS-CoV-2 Variants

  • 1. Institute for Physical Chemistry, Department of Chemistry, University of Basel, Basel 4058, Switzerland
  • 2. Department of Biosystems Science and Engineering, ETH Zurich, Basel 4058, Switzerland
  • 3. Department of Biomedicine, University of Basel, Basel 4056, Switzerland
  • 4. Department of Internal Medicine, University Hospital Basel, Basel 4056, Switzerland,
  • 5. Department of Internal Medicine, University Hospital Basel, Basel 4056, Switzerland

Description

Experimental data and scripts used for the development of a Yeast-based serological Assay:

  1. The Python scripts folder includes all scripts used in this study to analyze sera titers and plotting data.
  2. The Raw data folder includes:
    1. The Yeast Immunoassay development folder includes the flow cytometric data for serum depletion and reduction of ligand depletion effects.
    2. The Yeast serological data folder includes the raw data of all tested sera and related data for their analyses.
  3. The Snapgene plasmid maps folder includes the plasmid maps for the three SARS-CoV-2 VOCs for Snapgene.

 

 

Files

Python Scripts.zip

Files (1.4 MB)

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