Published March 15, 2023
| Version v1
Dataset
Open
Data for: Low protease activity in B cell follicles promotes retention of intact antigens after immunization
Authors/Creators
-
Aung, Aereas1
-
Cui, Ang2
-
Maiorino, Laura1
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Amini, Ava P.1
- Gregory, Justin R.1
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Bukenya, Maurice1
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Zhang, Yiming1
- Lee, Heya1
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Cottrell, Christopher A.3
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Morgan, Duncan M.1
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Silva, Murillo1
- Suh, Heikyung1
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Kirkpatrick, Jesse D.1
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Amlashi, Parastoo1
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Remba, Tanaka1
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Froehle, Leah M.4
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Xiao, Shuhao1
- Abraham, Wuhbet1
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Adams, Josetta1
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Love, J. Christopher1
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Huyett, Phillip5
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Kwon, Douglas S.4
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Hacohen, Nir2
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Schief, William R.3
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Bhatia, Sangeeta N.1
-
Irvine, Darrell J.1
- 1. Massachusetts Institute of Technology
- 2. Broad Institute
- 3. Scripps Research Institute
- 4. Ragon Institute of MGH, MIT and Harvard
- 5. Massachusetts Eye and Ear Infirmary
Description
The structural integrity of vaccine antigens is critical to the generation of protective antibody responses, but the impact of protease activity on vaccination in vivo is poorly understood. We characterized protease activity in lymph nodes and found that antigens were rapidly degraded in the subcapsular sinus, paracortex, and interfollicular regions, whereas low protease activity and antigen degradation rates were detected in the vicinity of follicular dendritic cells (FDCs). Correlated with these findings, immunization regimens designed to target antigen to FDCs led to germinal centers dominantly targeting intact antigen, whereas traditional immunizations led to much weaker responses that equally targeted the intact immunogen and antigen breakdown products. Thus, spatially compartmentalized antigen proteolysis affects humoral immunity and can be exploited.
Notes
The files are in "tif" format and can be opened with any image viewer.
Funding provided by: National Institute of Allergy and Infectious Diseases
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000060
Award Number: HIV/AIDS Vaccine Development Grant UM1 AI144462
Files
Fig1E_Outside_Follicles_6hrs_Antigen_Signal.tif
Files
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Additional details
Related works
- Is cited by
- 10.1101/2021.11.15.468669 (DOI)