Investigation of phytochemicals and determination of compounds of Phyllanthus emblica Linn. and Citrus limon (L.) Burm. through proximate analysis and In-vitro antimicrobial activity against pathogenic fungi Aspergillus flavus

Phyllanthus emblica and Citrus limon are medicinal plants usually found throughout India and its neighboring countries used in traditional medicine. The present study amid to evaluate compounds present in fruits of these two species through proximate analysis method, qualitative phytochemical analysis of methanol and hexane extracts of Phyllanthus emblica and Citrus limon. Tannin, flavanoid, alkaloid, terpenoid, glycoside, saponin, carbohydrate, protein and resin, were screened in the present study. The plant extracts of these two species were evaluated for their antifungal activity against, Aspergillus flavus, by using agar well diffusion method. Results of the study showed that hexane extract of Phyllanthus emblica was able to provide a maximum zone of inhibition (8.60±0.95) against Aspergillus flavus on the third day of inoculation, whereas hexane extract of Citrus limon was given least zone of inhibition (3.07±0.27) on the third day at 50 mg/ml.The plant extracts utilized in the present study exhibit the presence of assorted active components, at the side of their antioxidant and antimicrobial activities which can be helpful against totally different infections or diseases. The methanol and binary compound fruit extracts possess effective repressive activity against the tested pathogens. Additional identification and purification of active chemical constituents from the crude extracts of such medicative plants are going to be useful to develop a drug against pathogenic fungi.