Dataset Open Access
Recombinant Expression and Purification of ABHD2 from E. coli
Madison Edwards;
Levon Halabelian;
Aled Edwards;
Oliver Arnolds;
Opher Gileadi;
Peter Loppnau;
Alma Seitova
α/β Hydrolase domain-containing protein 2 (ABHD2), is a member of the metabolic serine hydrolases superfamily & a progesterone membrane receptor. In sperm, it supports the activation of CatSper which leads to sperm hypermotility. ABHD2 is 425 aa and has a N-terminal transmembrane domain followed by an extracellular facing domain. Here we report the recombinant expression and purification of ABHD2 amino acids 33-435 from E. coli. To the best of our knowledge, we are the first to have recombinantly purified active ABHD2.
Files
(6.1 MB)
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ABHD2 Purification nickel + SEC for open lab notebooks.docx
md5:babc67add2edfb51938079e0532cb0da |
6.1 MB | Download |
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Indexed in
- Publication date:
- March 3, 2023
- DOI:
-
- Keyword(s):
- ABHD2
- License (for files):
- Creative Commons Attribution 4.0 International