Dataset Open Access
Yaniv, Ziv; Anidi, Ifeanyichukwu; Arakkal, Leanne; Arroyo-Mejias, Armando; Beuschel, Rebecca T.; Börner, Katy; Chu, Colin J.; Clatworthy, Menna; Colautti, Jake; Croteau, Joshua; Dutra, Walderez; Fullam, Spencer; Gerner, Michael; Gola, Anita; Gollob, Kenneth J.; Ichise, Hiroshi; Jonigk, Danny; Kandov, Evelyn; Kastenmüller, Wolfgang; Koenig, Joshua F. E.; Murakami Olyntho, Vitoria; Padhan, Kartika; Richoz, Nathan; Shih, Rochelle; Speranza, Emily; Teichmann, Sarah; Veres, Tibor Z.; Vierhout, Megan; Wachter, Brianna; Williams, Margaret; Germain, Ronald N.; Radtke, Andrea J.
The Iterative Bleaching Extends Multiplexity (IBEX) imaging method is an iterative immunolabeling and chemical bleaching method that enables highly multiplexed imaging of diverse tissues. Development of the IBEX method and related software was led by Dr. Andrea Radtke and Dr. Ziv Yaniv. IBEX and related methods, Ce3D, Ce3D-IBEX, Opal-plex, were originally developed in the laboratory of Dr. Ronald N. Germain, US National Institutes of Health.
The IBEX Imaging Community is an international group of scientists committed to sharing knowledge related to multiplexed imaging in a transparent and collaborative manner. This open, global repository is a central resource for reagents, protocols, panels, publications, software, and datasets. In addition to IBEX, we support standard, single cycle multiplexed imaging (Multiplexed 2D imaging), volume imaging of cleared tissues with clearing enhanced 3D (Ce3D), highly multiplexed 3D imaging (Ce3D-IBEX), and extension of the IBEX dye inactivation protocol to the Leica Cell DIVE (Cell DIVE-IBEX). This dataset contains the current state of knowledge with respect to the IBEX microscopy imaging protocol.
How to use the Knowledge-Base:
Join the online IBEX Imaging community and contribute your knowledge. For more details on how to contribute, see these instructions.
This research was supported by:
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