Published February 24, 2023 | Version v1
Dataset Open

MinION plasmid deep long read sequencing for sequence verification

Authors/Creators

  • 1. Canada's Michael Smith Genome Sciences Centre

Description

We sequenced four plasmid constructs, each with a whole MinION flowcell, for use in developing and testing a sequence verification procedure. The resulting pipeline can sequence verify plasmid constructs, generating a consensus sequence with associated confidence of each base, adhering to strict acceptance criteria . The data contained herein are a subset of the complete data; 30 fast5 files for each plasmid, to be used as example data.

 

datHL_001519: BCRxV.TF.1
datHL_001521: BCRxV.VSVG.1
datHL_001617: BCRxV.GagPolRev.1
datHL_001620: BCRxV.VSVG.1_mutant

Files

Files (25.8 GB)