Images of H3K9me3 Immunostain in IMR90 Cells

Files are two-channel images at 49 unique xy positions showing the fluorescence intensity of a DRAQ5 DNA stain (c1) and H3K9me3 immunostain with an Alexa488 secondary (c2). Imaging was performed using a Nikon Ti2-E inverted microscope equipped with a Yokogawa CSU-W1 confocal spinning disk unit and a Zyla 2.3 PLUS sCMOS camera. We used 488 nm and 647 nm laser lines paired with 525/36 (MVI, 77074803), and 705/72 (MVI, 77074329) emission filters, respectively. Samples were imaged through a 1.15 NA CFI Apo LWD Lambda S 40X water immersion objective lens (Nikon MRD77410). The imagining system was controlled by NIS-Elements AR software.