Targeted Illumination in HeLa Cells

Files are a time series of showing targeted illumination of a subset of HeLa cell nuclei containing a photolabile ATAC-seq library. Time points are 300 ms apart. The single-channel images were acquired using a spinning disk confocal with a 40X objective (Nikon MRD77410) using a 561 nm laser line and 525/36 emission filter (MVI, 77074803). Images were captured using a Zyla 2.3 PLUS sCMOS camera. The pixel value represents the fluorescence intensity of the photocleavable fluorophore attached to the fragment library. Images are acquired concurrently with targeted illumination using a 405 nm laser line.