A COMPARATIVE ANALYSIS OF AZADIRACHTA INDICA LEAVES FROM DIFFERENT STATES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND ITS ANTIMICROBIAL PROPERTIES INVESTIGATION

In the present work, Neem leaf extracts from Maharashtra and Telangana were compared by using RP-HPLC for difference in chemical concentration. The chromatographic conditions were successfully developed for the separation of Maharashtra extract by using Kromosil RPC18 4.6×150mm 5µm, flow rate was 1.0ml/min, mobile phase ratio was ACE: methanol (75:35% v/v) pH-3 (pH was adjusted with Orthophosphoric acid), detection wavelength was 260 nm. The instrument used was WATERS HPLC Auto Sampler, Separation module 2690, photo diode array detector 996, Empower-software version-2. The retention times were found to be 2.223 min and 2.324 min. The chromatographic conditions were success fully developed for the separation of Telangana extract by using Agilent C 18(4.6×150mm) 5µ, flow rate was 1.0 ml/min, mobile phase ratio was Methanol: ACN (60:40% v/v) pH 3 (pH was adjusted with orthophosphoricacid), detection wavelength was 265nm. The instrument used was WATERS HPLC Auto Sampler, Separation module 2695, photo diode array detector 996, Empower-software version-2. The retention times were found to be 2.229 min and 3.330 min. The  results  of  the anti microbial activity studies suggest  that  the  samples from Maharashtra and Telangana  have  a  broad spectrum  of  antimicrobial  activity  and  this  effect  is  increased  by  increasing  the  quantity  of  this  compound, which can be used as an alternative for antibiotics.

KEYWORDS: Antibacterial activity, Azadirachta Indica, RP-HPLC, etc.