3D images of ovule primordia development in Zea mays - Part III

This image resource describes the development of ovule primordia of Zea mays, in 3D and at cellular resolution. The images were segmented using ImarisCell (Bitplane, Switzerland) and annotated as described in the associated manuscript Ouedraogo et al. (2023) Frontiers in Plant Science. DOI: 10.3389/fpls.2023.1174171

Zea mays (B73 genotype) floret meristems and ovule primordia were stained using cell wall and nuclear dyes, cleared using Clearsee solution (Kurihara et al., 2015), imaged using multiphoton microscopy and processed for segmentation in ImarisCell (Bitplane) as decribed in Mendocilla Sato et al., 2017. The .ims files also contains Surfaces objects for 3D rendering of specific cells or organs, used for quantitative analysis as described in the associated manuscript.

Each file corresponds to an individual ovule primordia or floret meristem, with file names corresponding to  ovule ID as described in Supplementary Dataset 1 of the associated manuscript Ouedraogo et al. (2023). Frontiers in Plant Science. DOI: 10.3389/fpls.2023.1174171