Published February 19, 2023 | Version v2
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CRISPR/Cas9 mediated knockout of ABCC1 confers resistance to Bacillus thuringiensis toxin Cry2Ab in Helicoverpa armigera

Authors/Creators

  • 1. Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China

Description

This data contains the Bt and insecticide bioassay results of ABCC1 knockout and wild-type strain of Helicoverpa armigera. Bioassay results revealed that the HaABCC1-KO strain exhibited >60-fold resistance to Cry2Ab toxin compared with the susceptible XJ strain, while no resistance has been recorded for Cry1Ab, Cry1Ac and Vip3A. Furthermore, the inheritance of H. armigera resistance to Cry2Ab was autosomal and completely recessive, significantly associated with the 4-nt deletion mutation of ABCC1 in the HaABCC1-KO strain. The relative growth of HaABCC1-KO and XJ strain also confirmed the role of HaABCC1 on the toxicity of Cry2Ab.

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